› 2bnj

X-ray diffraction
1.6Å resolution

The xylanase TA from Thermoascus aurantiacus utilizes arabinose decorations of xylan as significant substrate specificity determinants.

Released:
DOI: 10.2210/pdb2bnj/pdb
PDB entry 2bnj coloured by chain, front view.
PDB entry 2bnj coloured by chain, side view.
PDB entry 2bnj coloured by chain, top view.
Source organism: Thermoascus aurantiacus
Primary publication:
A family 10 Thermoascus aurantiacus xylanase utilizes arabinose decorations of xylan as significant substrate specificity determinants.
Vardakou M, Flint J, Christakopoulos P, Lewis RJ, Gilbert HJ, Murray JW
J Mol Biol 352 1060-7 (2005)
PMID: 16140328

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149932 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endo-1,4-beta-xylanase Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 32.85 KDa
Source organism: Thermoascus aurantiacus
UniProt:
  • Canonical: P23360 (Residues: 27-329; Coverage: 100%)
Gene name: XYNA
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP, AHR
Carbohydrate polymer
1 bound ligand:
Ligand FER 1 x FER
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 88.224Å b: 49.462Å c: 77.384Å
α: 90° β: 125.38° γ: 90°
R-values:
R R work R free
0.117 0.115 0.147

Quick links

Citations

5 review citations

Bioethanol.
Gray et al. (2006)
4 more

1 mention without citation

Insight into the cold adaptation and hemicellulose utilization of Cladosporium neopsychrotolerans from genome analysis and biochemical characterization.
Ma et al. (2018)