› 2bo6

X-ray diffraction
2.45Å resolution

DISSECTION OF MANNOSYLGLYCERATE SYNTHASE: AN ARCHETYPAL MANNOSYLTRANSFERASE

Released:
DOI: 10.2210/pdb2bo6/pdb
PDB entry 2bo6 coloured by chain, front view.
PDB entry 2bo6 coloured by chain, side view.
PDB entry 2bo6 coloured by chain, top view.
Source organism: Rhodothermus marinus
Primary publication:
Structural dissection and high-throughput screening of mannosylglycerate synthase.
Flint J, Taylor E, Yang M, Bolam DN, Tailford LE, Martinez-Fleites C, Dodson EJ, Davis BG, Gilbert HJ, Davies GJ
Nat Struct Mol Biol 12 608-14 (2005)
PMID: 15951819

Function and Biology Details

Reaction catalysed: 
GDP-alpha-D-mannose + D-glycerate = GDP + 2-O-(alpha-D-mannopyranosyl)-D-glycerate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannosylglycerate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 397 amino acids
Theoretical weight: 46.19 KDa
Source organism: Rhodothermus marinus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RFR0 (Residues: 1-397; Coverage: 100%)
Gene name: mgs
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
Ligand DGY 2 x DGY
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 150.228Å b: 150.228Å c: 154.285Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.255 0.253 0.277
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
9 more

2 mentions without citation

Identifying unexpected therapeutic targets via chemical-protein interactome.
Yang et al. (2010)
1 more