› 2boe

X-ray diffraction
1.15Å resolution

Catalytic domain of endo-1,4-glucanase Cel6A mutant Y73S from Thermobifida fusca

Released:
DOI: 10.2210/pdb2boe/pdb
Model geometry
Fit model/data
PDB entry 2boe coloured by chain, front view.
PDB entry 2boe coloured by chain, side view.
PDB entry 2boe coloured by chain, top view.
Source organism: Thermobifida fusca
Primary publication:
Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion.
Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA
Biochemistry 44 12915-22 (2005)
PMID: 16185060

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150674 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase E-2 Chain: X
Molecule details ›
Chain: X
Length: 286 amino acids
Theoretical weight: 30.36 KDa
Source organism: Thermobifida fusca
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26222 (Residues: 32-317; Coverage: 70%)
Gene name: celB
Sequence domains: Glycosyl hydrolases family 6
Structure domains: 1, 4-beta cellobiohydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 43.043Å b: 66.188Å c: 81.046Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

26 citation in other articles

Structure elucidation and biosynthesis of fuscachelins, peptide siderophores from the moderate thermophile Thermobifida fusca.
Dimise et al. (2008)
25 more

2 mentions without citation

Cellulases: ambiguous nonhomologous enzymes in a genomic perspective.
Sukharnikov et al. (2011)
1 more