› 2bog

X-ray diffraction
1.04Å resolution

Catalytic domain of endo-1,4-glucanase Cel6A mutant Y73S from Thermobifida fusca in complex with methyl cellobiosyl-4-thio-beta- cellobioside

Released:
DOI: 10.2210/pdb2bog/pdb
PDB entry 2bog coloured by chain, front view.
PDB entry 2bog coloured by chain, side view.
PDB entry 2bog coloured by chain, top view.
Source organism: Thermobifida fusca
Primary publication:
Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion.
Larsson AM, Bergfors T, Dultz E, Irwin DC, Roos A, Driguez H, Wilson DB, Jones TA
Biochemistry 44 12915-22 (2005)
PMID: 16185060

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150674 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoglucanase E-2 Chain: X
Molecule details ›
Chain: X
Length: 286 amino acids
Theoretical weight: 30.36 KDa
Source organism: Thermobifida fusca
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26222 (Residues: 32-317; Coverage: 70%)
Gene name: celB
Sequence domains: Glycosyl hydrolases family 6
Structure domains: 1, 4-beta cellobiohydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: MGL, SGC, BGC
Ligand MGL 1 x MGL
Ligand SGC 1 x SGC
Ligand BGC 2 x BGC
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 43.041Å b: 66.537Å c: 81.432Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.165
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 citation in other articles

Incorporation of fungal cellulases in bacterial minicellulosomes yields viable, synergistically acting cellulolytic complexes.
Mingardon et al. (2007)
24 more

3 mentions without citation

Discovery and characterization of a thermostable two-domain GH6 endoglucanase from a compost metagenome.
Jensen et al. (2018)
2 more