› 2bon

X-ray diffraction
1.9Å resolution

Structure of an Escherichia coli lipid kinase (YegS)

Released:
DOI: 10.2210/pdb2bon/pdb
PDB entry 2bon coloured by chain, front view.
PDB entry 2bon coloured by chain, side view.
PDB entry 2bon coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Crystal structure of YegS, a homologue to the mammalian diacylglycerol kinases, reveals a novel regulatory metal binding site.
Bakali HM, Herman MD, Johnson KA, Kelly AA, Wieslander A, Hallberg BM, Nordlund P
J Biol Chem 282 19644-52 (2007)
PMID: 17351295

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipid kinase YegS Chains: A, B
Molecule details ›
Chains: A, B
Length: 332 amino acids
Theoretical weight: 35.93 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76407 (Residues: 1-299; Coverage: 100%)
Gene names: JW2070, b2086, yegS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 42.326Å b: 166.164Å c: 48.474Å
α: 90° β: 97.03° γ: 90°
R-values:
R R work R free
0.222 0.22 0.267
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Regulation of sphingosine kinase and sphingolipid signaling.
Pitson SM. (2011)
3 more

2 mentions without citation

Analysis of the Staphylococcus aureus DgkB structure reveals a common catalytic mechanism for the soluble diacylglycerol kinases.
Miller et al. (2008)
1 more