› 2boo

X-ray diffraction
1.8Å resolution

The crystal structure of Uracil-DNA N-Glycosylase (UNG) from Deinococcus radiodurans.

Released:
DOI: 10.2210/pdb2boo/pdb
PDB entry 2boo coloured by chain, front view.
PDB entry 2boo coloured by chain, side view.
PDB entry 2boo coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
Structure of the uracil-DNA N-glycosylase (UNG) from Deinococcus radiodurans.
Leiros I, Moe E, Smalås AO, McSweeney S
Acta Crystallogr D Biol Crystallogr 61 1049-56 (2005)
PMID: 16041069

Function and Biology Details

Reaction catalysed: 
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 247 amino acids
Theoretical weight: 27.78 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RWH9 (Residues: 1-247; Coverage: 100%)
Gene names: DR_0689, ung
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain

Ligands and Environments

1 bound ligand:
Ligand NO3 3 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 63.5Å b: 85.1Å c: 85.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.234 0.272
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Deinococcus radiodurans: what belongs to the survival kit?
Blasius et al. (2008)
1 more

1 mention without citation

Protein surface characterization using an invariant descriptor.
Abu Deeb et al. (2011)