› 2bop

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE AT 1.7 ANGSTROMS OF THE BOVINE PAPILLOMAVIRUS-1 E2 DNA-BINDING DOMAIN BOUND TO ITS DNA TARGET

Released:
DOI: 10.2210/pdb2bop/pdb
PDB entry 2bop coloured by chain, front view.
PDB entry 2bop coloured by chain, side view.
PDB entry 2bop coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure at 1.7 A of the bovine papillomavirus-1 E2 DNA-binding domain bound to its DNA target.
Hegde RS, Grossman SR, Laimins LA, Sigler PB
Nature 359 505-12 (1992)
PMID: 1328886

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115119 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Regulatory protein E2 Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 9.62 KDa
Source organism: Deltapapillomavirus 4
Expression system: Escherichia coli
UniProt:
  • Canonical: P03122 (Residues: 326-410; Coverage: 21%)
Gene name: E2
Sequence domains: E2 (early) protein, C terminal
Structure domains: Alpha-Beta Plaits
DNA (5'-D(*CP*CP*GP*AP*CP*CP*GP*AP*CP*GP*TP*CP*GP*GP*TP*CP*G )-3') Chain: B
Molecule details ›
Chain: B
Length: 17 nucleotides
Theoretical weight: 5.19 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand YB 2 x YB
No modified residues

Experiments and Validation Details

Entry percentile scores
Unit cell:
a: 64.6Å b: 64.6Å c: 64.6Å
α: 61.8° β: 61.8° γ: 61.8°
R-values:
R R work R free
0.201 not available not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

40 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
39 more

22 mentions without citation

The papillomavirus E2 proteins.
McBride AA. (2013)
21 more