› 2boy

X-ray diffraction
1.9Å resolution

Crystal structure of 3-ChloroCatechol 1,2-Dioxygenase from Rhodococcus Opacus 1CP

Released:
DOI: 10.2210/pdb2boy/pdb
PDB entry 2boy coloured by chain, front view.
PDB entry 2boy coloured by chain, side view.
PDB entry 2boy coloured by chain, top view.
Source organism: Rhodococcus opacus
Primary publication:
Crystal structure of 3-chlorocatechol 1,2-dioxygenase key enzyme of a new modified ortho-pathway from the Gram-positive Rhodococcus opacus 1CP grown on 2-chlorophenol.
Ferraroni M, Kolomytseva MP, Solyanikova IP, Scozzafava A, Golovleva LA, Briganti F
J Mol Biol 360 788-99 (2006)
PMID: 16793061

Function and Biology Details

Reaction catalysed: 
Catechol + O(2) = cis,cis-muconate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184471 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Intradiol ring-cleavage dioxygenases domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 254 amino acids
Theoretical weight: 28.13 KDa
Source organism: Rhodococcus opacus
UniProt:
  • Canonical: Q8G9L3 (Residues: 1-254; Coverage: 100%)
Gene names: R1CP_39610, clcA2, clcA3
Sequence domains:
Structure domains: Aromatic compound dioxygenase

Ligands and Environments

4 bound ligands:
Ligand FE 8 x FE
Ligand BHO 8 x BHO
Ligand LPP 8 x LPP
Ligand MG 16 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P1
Unit cell:
a: 83.179Å b: 86.61Å c: 93.447Å
α: 85.37° β: 66.53° γ: 76.94°
R-values:
R R work R free
0.178 0.176 0.219

Quick links

Citations

1 review citation

Biological Inspirations: Iron Complexes Mimicking the Catechol Dioxygenases.
Kałduńska et al. (2021)
12 other articles

5 mentions without citation

Empirical power laws for the radii of gyration of protein oligomers.
Tanner JJ. (2016)
4 more