› 2bp8

X-ray diffraction
1.9Å resolution

M144Q Structure of nitrite reductase from Alcaligenes xylosoxidans

Released:
DOI: 10.2210/pdb2bp8/pdb
PDB entry 2bp8 coloured by chain, front view.
PDB entry 2bp8 coloured by chain, side view.
PDB entry 2bp8 coloured by chain, top view.
Source organism: Achromobacter xylosoxidans
Primary publication:
High resolution structural studies of mutants provide insights into catalysis and electron transfer processes in copper nitrite reductase.
Hough MA, Ellis MJ, Antonyuk S, Strange RW, Sawers G, Eady RR, Samar Hasnain S
J Mol Biol 350 300-9 (2005)
PMID: 15927201

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130622 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Copper-containing nitrite reductase Chain: A
Molecule details ›
Chain: A
Length: 336 amino acids
Theoretical weight: 36.57 KDa
Source organism: Achromobacter xylosoxidans
Expression system: Escherichia coli
UniProt:
  • Canonical: O68601 (Residues: 25-360; Coverage: 100%)
Gene names: O9570_16215, nir, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins
Copper-containing nitrite reductase Chain: B
Molecule details ›
Chain: B
Length: 336 amino acids
Theoretical weight: 36.57 KDa
Source organism: Achromobacter xylosoxidans
Expression system: Escherichia coli
UniProt:
  • Canonical: O68601 (Residues: 25-360; Coverage: 100%)
Gene names: O9570_16215, nir, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
Ligand CU 4 x CU
Ligand ZN 10 x ZN
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: R3
Unit cell:
a: 90.6Å b: 90.6Å c: 291.458Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.149 0.189
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
2 more