› 2bpa

X-ray diffraction
3Å resolution

ATOMIC STRUCTURE OF SINGLE-STRANDED DNA BACTERIOPHAGE PHIX174 AND ITS FUNCTIONAL IMPLICATIONS

Released:
DOI: 10.2210/pdb2bpa/pdb
PDB entry 2bpa coloured by chain, front view.
PDB entry 2bpa coloured by chain, side view.
PDB entry 2bpa coloured by chain, top view.
Source organism: Sinsheimervirus phiX174
Primary publication:
Atomic structure of single-stranded DNA bacteriophage phi X174 and its functional implications.
McKenna R, Xia D, Willingmann P, Ilag LL, Krishnaswamy S, Rossmann MG, Olson NH, Baker TS, Incardona NL
Nature 355 137-43 (1992)
PMID: 1370343

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero 240-mer (preferred)
PDBe Complex ID:
PDB-CPX-115120 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (4 distinct):
Capsid protein F Chain: 1
Molecule details ›
Chain: 1
Length: 426 amino acids
Theoretical weight: 48.42 KDa
Source organism: Sinsheimervirus phiX174
Expression system: Escherichia coli C
UniProt:
  • Canonical: P03641 (Residues: 2-427; Coverage: 100%)
Gene name: F
Sequence domains: Capsid protein (F protein)
Structure domains: Microviridae F protein
Major spike protein G Chain: 2
Molecule details ›
Chain: 2
Length: 175 amino acids
Theoretical weight: 19.06 KDa
Source organism: Sinsheimervirus phiX174
Expression system: Escherichia coli C
UniProt:
  • Canonical: P03643 (Residues: 1-175; Coverage: 100%)
Gene name: G
Sequence domains: Major spike protein (G protein)
Structure domains: Jelly Rolls
DNA-binding protein J Chain: 3
Molecule details ›
Chain: 3
Length: 37 amino acids
Theoretical weight: 4.11 KDa
Source organism: Sinsheimervirus phiX174
Expression system: Escherichia coli C
UniProt:
  • Canonical: P69592 (Residues: 2-38; Coverage: 97%)
Gene name: J
Sequence domains: Microvirus J protein
DNA (5'-D(*AP*AP*AP*AP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 5 nucleotides
Theoretical weight: 1.5 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 305.58Å b: 360.78Å c: 299.46Å
α: 90° β: 92.89° γ: 90°
R-values:
R R work R free
0.209 not available not available
Expression system: Escherichia coli C

Quick links

Citations

11 review citations

Phage diversity, genomics and phylogeny.
Dion et al. (2020)
10 more

21 mentions without citation

Virus Isoelectric Point Estimation: Theories and Methods.
Heffron et al. (2021)
20 more