› 2bqy

X-ray diffraction
2.3Å resolution

Inorganic Pyrophosphatase from the Pathogenic Bacterium Helicobacter pylori-Kinetic and Structural Properties

Released:
DOI: 10.2210/pdb2bqy/pdb
PDB entry 2bqy coloured by chain, front view.
PDB entry 2bqy coloured by chain, side view.
PDB entry 2bqy coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Kinetic and structural properties of inorganic pyrophosphatase from the pathogenic bacterium Helicobacter pylori.
Chao TC, Huang H, Tsai JY, Huang CY, Sun YJ
Proteins 65 670-80 (2006)
PMID: 16988955

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157400 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inorganic pyrophosphatase Chain: A
Molecule details ›
Chain: A
Length: 173 amino acids
Theoretical weight: 19.3 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: P56153 (Residues: 1-173; Coverage: 100%)
Gene names: HP_0620, ppa
Sequence domains: Inorganic pyrophosphatase
Structure domains: Inorganic pyrophosphatase

Ligands and Environments

1 bound ligand:
Ligand POP 1 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL17B2
Spacegroup: P6322
Unit cell:
a: 101.5Å b: 101.5Å c: 93.95Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.25 0.25 0.288
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

A novel fluorescence assay for inorganic pyrophosphatase based on modulated aggregation of graphene quantum dots.
Zhu et al. (2016)
5 more