› 2btf

X-ray diffraction
2.55Å resolution

THE STRUCTURE OF CRYSTALLINE PROFILIN-BETA-ACTIN

Released:
DOI: 10.2210/pdb2btf/pdb
PDB entry 2btf coloured by chain, front view.
PDB entry 2btf coloured by chain, side view.
PDB entry 2btf coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The structure of crystalline profilin-beta-actin.
Schutt CE, Myslik JC, Rozycki MD, Goonesekere NC, Lindberg U
Nature 365 810-6 (1993)
PMID: 8413665

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135936 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, cytoplasmic 1 Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.69 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P60712 (Residues: 2-375; Coverage: 100%)
Gene name: ACTB
Sequence domains: Actin
Structure domains:
Profilin-1 Chain: P
Molecule details ›
Chain: P
Length: 140 amino acids
Theoretical weight: 14.97 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P02584 (Residues: 2-140; Coverage: 99%)
Gene name: PFN1
Sequence domains: Profilin
Structure domains: Dynein light chain 2a, cytoplasmic

Ligands and Environments

2 bound ligands:
Ligand SR 1 x SR
Ligand ATP 1 x ATP
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 38.95Å b: 71.3Å c: 171.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 not available
Expression system: Not provided

Quick links

Citations

82 review citations

Molecular mechanisms controlling actin filament dynamics in nonmuscle cells.
Pollard et al. (2000)
81 more

96 mentions without citation

The state of the filament.
Aguda et al. (2005)
95 more