› 2btq

X-ray diffraction
3.2Å resolution

Structure of BtubAB heterodimer from Prosthecobacter dejongeii

Released:
DOI: 10.2210/pdb2btq/pdb
PDB entry 2btq coloured by chain, front view.
PDB entry 2btq coloured by chain, side view.
PDB entry 2btq coloured by chain, top view.
Source organism: Prosthecobacter dejongeii
Primary publication:
Structure of bacterial tubulin BtubA/B: evidence for horizontal gene transfer.
Schlieper D, Oliva MA, Andreu JM, Löwe J
Proc Natl Acad Sci U S A 102 9170-5 (2005)
PMID: 15967998

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184493 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tubulin/FtsZ GTPase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 473 amino acids
Theoretical weight: 51.3 KDa
Source organism: Prosthecobacter dejongeii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GCC5 (Residues: 1-473; Coverage: 100%)
Gene name: btubA
Sequence domains:
Structure domains:
Tubulin BtubB Chain: B
Molecule details ›
Chain: B
Length: 426 amino acids
Theoretical weight: 46.47 KDa
Source organism: Prosthecobacter dejongeii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GCC1 (Residues: 1-426; Coverage: 100%)
Gene name: btubB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6522
Unit cell:
a: 154.398Å b: 154.398Å c: 256.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.215 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

41 review citations

Horizontal gene transfer in eukaryotic evolution.
Keeling et al. (2008)
40 more

6 mentions without citation

Structure of bacterial tubulin BtubA/B: evidence for horizontal gene transfer.
Schlieper et al. (2005)
5 more