› 2bu1

X-ray diffraction
2.2Å resolution

MS2-RNA HAIRPIN (5BRU -5) COMPLEX

Released:
DOI: 10.2210/pdb2bu1/pdb
PDB entry 2bu1 coloured by chain, front view.
PDB entry 2bu1 coloured by chain, side view.
PDB entry 2bu1 coloured by chain, top view.
Source organism: Escherichia phage MS2
Primary publication:
Structural basis of pyrimidine specificity in the MS2 RNA hairpin-coat-protein complex.
Grahn E, Moss T, Helgstrand C, Fridborg K, Sundaram M, Tars K, Lago H, Stonehouse NJ, Davis DR, Stockley PG, Liljas L
RNA 7 1616-27 (2001)
PMID: 11720290

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137151 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Capsid protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 129 amino acids
Theoretical weight: 13.74 KDa
Source organism: Escherichia phage MS2
Expression system: Escherichia coli
UniProt:
  • Canonical: P03612 (Residues: 2-130; Coverage: 99%)
Sequence domains: Levivirus coat protein
Structure domains: MS2 Viral Coat Protein
5'-R(*AP*CP*AP*UP*GP*AP*GP*GP*AP*UP *5BU*AP*CP*CP*CP*AP*UP*GP*U)-3' Chains: R, S
Molecule details ›
Chains: R, S
Length: 19 nucleotides
Theoretical weight: 6.14 KDa
Source organism: Escherichia phage MS2
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 5BU 2 x 5BU

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: R32
Unit cell:
a: 288Å b: 288Å c: 653Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.219 0.244
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Recognition modes of RNA tetraloops and tetraloop-like motifs by RNA-binding proteins.
Thapar et al. (2014)
21 other articles

8 mentions without citation

Linking RNA Sequence, Structure, and Function on Massively Parallel High-Throughput Sequencers.
Denny et al. (2019)
7 more