› 2bu3

X-ray diffraction
1.4Å resolution

Acyl-enzyme intermediate between Alr0975 and glutathione at pH 3.4

Released:
DOI: 10.2210/pdb2bu3/pdb
PDB entry 2bu3 coloured by chain, front view.
PDB entry 2bu3 coloured by chain, side view.
PDB entry 2bu3 coloured by chain, top view.
Source organism: Anabaena sp.
Primary publication:
A papain-like enzyme at work: native and acyl-enzyme intermediate structures in phytochelatin synthesis.
Vivares D, Arnoux P, Pignol D
Proc Natl Acad Sci U S A 102 18848-53 (2005)
PMID: 16339904

Function and Biology Details

Reaction catalysed: 
Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187143 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glutathione gamma-glutamylcysteinyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 254 amino acids
Theoretical weight: 29.44 KDa
Source organism: Anabaena sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YY76 (Residues: 23-242; Coverage: 100%)
Gene name: alr0975
Sequence domains: Phytochelatin synthase
Structure domains: Phytochelatin synthase, N-terminal domain

Ligands and Environments

3 bound ligands:
Ligand 3GC 2 x 3GC
Ligand CL 2 x CL
Ligand CA 2 x CA
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 56.253Å b: 58.259Å c: 72.561Å
α: 90° β: 108.87° γ: 90°
R-values:
R R work R free
0.174 0.174 0.188
Expression system: Escherichia coli

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Citations

4 review citations

Structural biology of plant sulfur metabolism: from assimilation to biosynthesis.
Ravilious et al. (2012)
3 more

7 mentions without citation

A bacterial type III effector family uses the papain-like hydrolytic activity to arrest the host cell cycle.
Yao et al. (2009)
6 more