› 2bun

Solution NMR

Solution structure of the BLUF domain of AppA 5-125

Released:
DOI: 10.2210/pdb2bun/pdb
PDB entry 2bun coloured by chain, ensemble of 20 models, front view.
PDB entry 2bun coloured by chain, ensemble of 20 models, side view.
PDB entry 2bun coloured by chain, ensemble of 20 models, top view.
Source organism: Cereibacter sphaeroides 2.4.1
Primary publication:
The solution structure of the AppA BLUF domain: insight into the mechanism of light-induced signaling.
Grinstead JS, Hsu ST, Laan W, Bonvin AM, Hellingwerf KJ, Boelens R, Kaptein R
Chembiochem 7 187-93 (2006)
PMID: 16323221

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176041 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BLUF domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 13.51 KDa
Source organism: Cereibacter sphaeroides 2.4.1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53119 (Residues: 5-125; Coverage: 27%)
Gene name: appA
Sequence domains: Sensors of blue-light using FAD
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand FAD 1 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: ARIA
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Structure and function of plant photoreceptors.
Möglich et al. (2010)
15 more

5 mentions without citation

Seeing the light with BLUF proteins.
Park et al. (2017)
4 more