› 2bv6

X-ray diffraction
2.8Å resolution

Crystal structure of MgrA, a global regulator and major virulence determinant in Staphylococcus aureus

Released:
DOI: 10.2210/pdb2bv6/pdb
PDB entry 2bv6 coloured by chain, front view.
PDB entry 2bv6 coloured by chain, side view.
PDB entry 2bv6 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
An oxidation-sensing mechanism is used by the global regulator MgrA in Staphylococcus aureus.
Chen PR, Bae T, Williams WA, Duguid EM, Rice PA, Schneewind O, He C
Nat Chem Biol 2 591-5 (2006)
PMID: 16980961

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142961 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator MgrA Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 16.53 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0C1S0 (Residues: 2-143; Coverage: 97%)
Gene names: mgr, mgrA
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P6122
Unit cell:
a: 119.8Å b: 119.8Å c: 62.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.25 0.25 0.292
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis.
D'Autréaux et al. (2007)
28 more

9 mentions without citation

From genotype to phenotype: can systems biology be used to predict Staphylococcus aureus virulence?
Priest et al. (2012)
8 more