› 2bvf

X-ray diffraction
1.92Å resolution

Crystal structure of 6-hydoxy-D-nicotine oxidase from Arthrobacter nicotinovorans. Crystal Form 3 (P1)

Released:
DOI: 10.2210/pdb2bvf/pdb
PDB entry 2bvf coloured by chain, front view.
PDB entry 2bvf coloured by chain, side view.
PDB entry 2bvf coloured by chain, top view.
Source organism: Paenarthrobacter nicotinovorans
Primary publication:
Crystal structure of 6-hydroxy-D-nicotine oxidase from Arthrobacter nicotinovorans.
Koetter JW, Schulz GE
J Mol Biol 352 418-28 (2005)
PMID: 16095622

Function and Biology Details

Reaction catalysed: 
(1a) (R)-6-hydroxynicotine + O(2) = 5-(N-methyl-4,5-dihydro-1H-pyrrol-2-yl)pyridin-2-ol + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139940 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(R)-6-hydroxynicotine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 459 amino acids
Theoretical weight: 48.92 KDa
Source organism: Paenarthrobacter nicotinovorans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08159 (Residues: 1-458; Coverage: 100%)
Gene name: 6-hdno
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 60.527Å b: 62.398Å c: 73.351Å
α: 89.56° β: 85.29° γ: 73.03°
R-values:
R R work R free
0.17 0.169 0.208
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Flavoenzymes.
Joosten et al. (2007)
6 more

4 mentions without citation

The enzymes of microbial nicotine metabolism.
Fitzpatrick PF. (2018)
3 more