› 2bx7

X-ray diffraction
2.04Å resolution

Crystal structure of L. lactis dihydroorotate dehydrogense A in complex with 3,5-dihydroxybenzoate

Released:
DOI: 10.2210/pdb2bx7/pdb
PDB entry 2bx7 coloured by chain, front view.
PDB entry 2bx7 coloured by chain, side view.
PDB entry 2bx7 coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
Interaction of benzoate pyrimidine analogues with class 1A dihydroorotate dehydrogenase from Lactococcus lactis.
Wolfe AE, Thymark M, Gattis SG, Fagan RL, Hu YC, Johansson E, Arent S, Larsen S, Palfey BA
Biochemistry 46 5741-53 (2007)
PMID: 17444658

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + fumarate = orotate + succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106948 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase A (fumarate) Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 34.24 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: A2RJT9 (Residues: 1-311; Coverage: 100%)
Gene names: llmg_0952, pyrDA
Sequence domains: Dihydroorotate dehydrogenase
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
4 bound ligands:
Ligand ACT 1 x ACT
Ligand MG 2 x MG
Ligand GOL 1 x GOL
Ligand 34D 1 x 34D
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 53.71Å b: 108.127Å c: 66.216Å
α: 90° β: 104.06° γ: 90°
R-values:
R R work R free
0.187 0.184 0.229
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Plasmodium dihydroorotate dehydrogenase: a promising target for novel anti-malarial chemotherapy.
Phillips et al. (2010)
1 more