› 2byc

X-ray diffraction
1.9Å resolution

BlrB - a BLUF protein, dark state structure

Released:
DOI: 10.2210/pdb2byc/pdb
PDB entry 2byc coloured by chain, front view.
PDB entry 2byc coloured by chain, side view.
PDB entry 2byc coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Structure of a bacterial BLUF photoreceptor: insights into blue light-mediated signal transduction.
Jung A, Domratcheva T, Tarutina M, Wu Q, Ko WH, Shoeman RL, Gomelsky M, Gardner KH, Schlichting I
Proc Natl Acad Sci U S A 102 12350-5 (2005)
PMID: 16107542

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174591 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BLUF domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 137 amino acids
Theoretical weight: 15.56 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3IYE4 (Residues: 1-136; Coverage: 97%)
Gene names: RSP_1261, blrB
Sequence domains: Sensors of blue-light using FAD
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand FMN 2 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 36.6Å b: 65Å c: 106.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.232 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Structure and function of plant photoreceptors.
Möglich et al. (2010)
18 more

9 mentions without citation

Photochemistry of flavoprotein light sensors.
Conrad et al. (2014)
8 more