› 2c0a

X-ray diffraction
1.55Å resolution

Mechanism of the Class I KDPG aldolase

Released:
DOI: 10.2210/pdb2c0a/pdb
PDB entry 2c0a coloured by chain, front view.
PDB entry 2c0a coloured by chain, side view.
PDB entry 2c0a coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Mechanism of the Class I KDPG aldolase.
Fullerton SW, Griffiths JS, Merkel AB, Cheriyan M, Wymer NJ, Hutchins MJ, Fierke CA, Toone EJ, Naismith JH
Bioorg Med Chem 14 3002-10 (2006)
PMID: 16403639

Function and Biology Details

Reactions catalysed: 
2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate
(4R)-4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
KHG/KDPG aldolase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 214 amino acids
Theoretical weight: 22.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A955 (Residues: 1-213; Coverage: 100%)
Gene names: JW1839, b1850, eda, hga, kdgA
Sequence domains: KDPG and KHG aldolase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 54.492Å b: 84.244Å c: 132.402Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
4 more

2 mentions without citation

Key Enzymes of the Semiphosphorylative Entner-Doudoroff Pathway in the Haloarchaeon Haloferax volcanii: Characterization of Glucose Dehydrogenase, Gluconate Dehydratase, and 2-Keto-3-Deoxy-6-Phosphogluconate Aldolase.
Sutter et al. (2016)
1 more