› 2c0z

X-ray diffraction
1.6Å resolution

The 1.6 A resolution crystal structure of NovW: a 4-keto-6-deoxy sugar epimerase from the novobiocin biosynthetic gene cluster of Streptomyces spheroides

Released:
DOI: 10.2210/pdb2c0z/pdb
PDB entry 2c0z coloured by chain, front view.
PDB entry 2c0z coloured by chain, side view.
PDB entry 2c0z coloured by chain, top view.
Source organism: Streptomyces niveus NCIMB 11891
Primary publication:
The 1.6-A resolution crystal structure of NovW: a 4-keto-6-deoxy sugar epimerase from the novobiocin biosynthetic gene cluster of Streptomyces spheroides.
Jakimowicz P, Tello M, Meyers CL, Walsh CT, Buttner MJ, Field RA, Lawson DM
Proteins 63 261-5 (2006)
PMID: 16411240

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dTDP-4-dehydrorhamnose 3-epimerase Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 23.2 KDa
Source organism: Streptomyces niveus NCIMB 11891
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9L9E5 (Residues: 1-207; Coverage: 100%)
Gene name: novW
Sequence domains: dTDP-4-dehydrorhamnose 3,5-epimerase
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P43212
Unit cell:
a: 59.354Å b: 59.354Å c: 109.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.195
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

The structural biology of enzymes involved in natural product glycosylation.
Singh et al. (2012)
3 more

3 mentions without citation

Structure-based phylogeny as a diagnostic for functional characterization of proteins with a cupin fold.
Agarwal et al. (2009)
2 more