› 2c1h

X-ray diffraction
2.6Å resolution

The X-ray Structure of Chlorobium vibrioforme 5-Aminolaevulinic Acid Dehydratase Complexed with a Diacid Inhibitor

Released:
DOI: 10.2210/pdb2c1h/pdb
PDB entry 2c1h coloured by chain, front view.
PDB entry 2c1h coloured by chain, side view.
PDB entry 2c1h coloured by chain, top view.
Source organism: Prosthecochloris vibrioformis
Primary publication:
Structure of Chlorobium vibrioforme 5-aminolaevulinic acid dehydratase complexed with a diacid inhibitor.
Coates L, Beaven G, Erskine PT, Beale SI, Wood SP, Shoolingin-Jordan PM, Cooper JB
Acta Crystallogr D Biol Crystallogr 61 1594-8 (2005)
PMID: 16304458

Function and Biology Details

Reaction catalysed: 
2 5-aminolevulinate = porphobilinogen + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-aminolevulinic acid dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 36.43 KDa
Source organism: Prosthecochloris vibrioformis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59334 (Residues: 1-328; Coverage: 100%)
Gene names: Cpar_0732, hemB
Sequence domains: Delta-aminolevulinic acid dehydratase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand DSB 2 x DSB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P4212
Unit cell:
a: 126.48Å b: 126.48Å c: 81.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.263 0.26 0.32
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.
Dailey et al. (2017)
1 more

2 mentions without citation

Crystal structure of Toxoplasma gondii porphobilinogen synthase: insights on octameric structure and porphobilinogen formation.
Jaffe et al. (2011)
1 more