› 2c1w

X-ray diffraction
2.2Å resolution

The structure of XendoU: a splicing independent snoRNA processing endoribonuclease

Released:
DOI: 10.2210/pdb2c1w/pdb
PDB entry 2c1w coloured by chain, front view.
PDB entry 2c1w coloured by chain, side view.
PDB entry 2c1w coloured by chain, top view.
Source organism: Xenopus laevis
Primary publication:
The structure of the endoribonuclease XendoU: From small nucleolar RNA processing to severe acute respiratory syndrome coronavirus replication.
Renzi F, Caffarelli E, Laneve P, Bozzoni I, Brunori M, Vallone B
Proc Natl Acad Sci U S A 103 12365-70 (2006)
PMID: 16895992

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184996 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(U)-specific endoribonuclease-A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 292 amino acids
Theoretical weight: 33.91 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8JFY9 (Residues: 1-292; Coverage: 100%)
Gene name: endou-a
Sequence domains: Endoribonuclease XendoU

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 164.451Å b: 53.204Å c: 133.466Å
α: 90° β: 121.86° γ: 90°
R-values:
R R work R free
0.274 0.274 0.277
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
9 more

12 mentions without citation

Information available at cut rates: structure and mechanism of ribonucleases.
Worrall et al. (2007)
11 more