› 2c2a

X-ray diffraction
1.9Å resolution

Structure of the entire cytoplasmic portion of a sensor histidine kinase protein

Released:
DOI: 10.2210/pdb2c2a/pdb
PDB entry 2c2a coloured by chain, front view.
PDB entry 2c2a coloured by chain, side view.
PDB entry 2c2a coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structure of the entire cytoplasmic portion of a sensor histidine-kinase protein.
Marina A, Waldburger CD, Hendrickson WA
EMBO J 24 4247-59 (2005)
PMID: 16319927

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194642 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 29.41 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WZV7 (Residues: 232-489; Coverage: 53%)
Gene name: TM_0853
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 79.292Å b: 162.082Å c: 42.525Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.246 0.275
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Specificity in two-component signal transduction pathways.
Laub et al. (2007)
24 more

72 mentions without citation

Diversity of structure and function of response regulator output domains.
Galperin MY. (2010)
71 more