› 2c2x

X-ray diffraction
2Å resolution

Three dimensional structure of bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb2c2x/pdb
PDB entry 2c2x coloured by chain, front view.
PDB entry 2c2x coloured by chain, side view.
PDB entry 2c2x coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Javid-Majd F, Sacchettini JC, TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Reactions catalysed: 
5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161467 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein FolD Chains: A, B
Molecule details ›
Chains: A, B
Length: 281 amino acids
Theoretical weight: 29.49 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WG81 (Residues: 1-281; Coverage: 100%)
Gene names: Rv3356c, folD
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 59.511Å b: 86.76Å c: 111.539Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.248
Expression system: Escherichia coli BL21(DE3)

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