› 2c4r

X-ray diffraction
3.6Å resolution

Catalytic domain of E. coli RNase E

Released:
DOI: 10.2210/pdb2c4r/pdb
PDB entry 2c4r coloured by chain, front view.
PDB entry 2c4r coloured by chain, side view.
PDB entry 2c4r coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of Escherichia coli RNase E catalytic domain and implications for RNA turnover.
Callaghan AJ, Marcaida MJ, Stead JA, McDowall KJ, Scott WG, Luisi BF
Nature 437 1187-91 (2005)
PMID: 16237448

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149275 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease E Chain: L
Molecule details ›
Chain: L
Length: 517 amino acids
Theoretical weight: 58.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21513 (Residues: 1-510; Coverage: 48%)
Gene names: JW1071, ams, b1084, hmp1, rne
Sequence domains:
Structure domains: Ribonuclease E, catalytic domain
SSRNA MOLECULE: 5'-R(*AP*CP*AP*GP*UP*AP*UP*UP*UP*GP)-3' Chain: R
Molecule details ›
Chain: R
Length: 10 nucleotides
Theoretical weight: 3.16 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS
Spacegroup: P6222
Unit cell:
a: 196.586Å b: 196.586Å c: 140.766Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.32 0.319 0.347
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

42 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
41 more

7 mentions without citation

Rapid cleavage of RNA by RNase E in the absence of 5' monophosphate stimulation.
Kime et al. (2010)
6 more