› 2c5e

X-ray diffraction
1.7Å resolution

gdp-mannose-3', 5' -epimerase (arabidopsis thaliana), k217a, with gdp-alpha-d-mannose bound in the active site.

Released:
DOI: 10.2210/pdb2c5e/pdb
PDB entry 2c5e coloured by chain, front view.
PDB entry 2c5e coloured by chain, side view.
PDB entry 2c5e coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structure and function of GDP-mannose-3',5'-epimerase: an enzyme which performs three chemical reactions at the same active site.
Major LL, Wolucka BA, Naismith JH
J Am Chem Soc 127 18309-20 (2005)
PMID: 16366586

Function and Biology Details

Reaction catalysed: 
GDP-alpha-D-mannose = GDP-beta-L-gulose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GDP-mannose 3,5-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 379 amino acids
Theoretical weight: 42.89 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93VR3 (Residues: 1-377; Coverage: 100%)
Gene names: At5g28840, F7P1.20
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand GDD 2 x GDD
Ligand FMT 10 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 62.481Å b: 83.617Å c: 66.232Å
α: 90° β: 99.01° γ: 90°
R-values:
R R work R free
0.134 0.131 0.193
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The structure, function, and biosynthesis of plant cell wall pectic polysaccharides.
Caffall et al. (2009)
9 more

3 mentions without citation

GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial Potential.
Beerens et al. (2021)
2 more