› 2c5k

X-ray diffraction
2.05Å resolution

N-terminal domain of tlg1 complexed with N-terminus of vps51

Released:
DOI: 10.2210/pdb2c5k/pdb
PDB entry 2c5k coloured by chain, front view.
PDB entry 2c5k coloured by chain, side view.
PDB entry 2c5k coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural analysis of the interaction between the SNARE Tlg1 and Vps51.
Fridmann-Sirkis Y, Kent HM, Lewis MJ, Evans PR, Pelham HR
Traffic 7 182-90 (2006)
PMID: 16420526

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153128 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vacuolar protein sorting-associated protein 51 Chain: P
Molecule details ›
Chain: P
Length: 24 amino acids
Theoretical weight: 2.97 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P36116 (Residues: 9-32; Coverage: 15%)
Gene names: API3, VPS51, VPS67, WHI6, YKR020W
t-SNARE affecting a late Golgi compartment protein 1 Chain: T
Molecule details ›
Chain: T
Length: 95 amino acids
Theoretical weight: 11.24 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q03322 (Residues: 1-95; Coverage: 42%)
Gene names: D8035.11, TLG1, YDR468C
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 60.452Å b: 60.452Å c: 62.388Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.175 0.221
Expression systems:
  • Not provided
  • Escherichia coli BL21

Quick links

Citations

10 review citations

Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle.
Cai et al. (2007)
9 more

6 mentions without citation

Structure of Golgi transport proteins.
Kümmel et al. (2011)
5 more