› 2c8c

X-ray diffraction
2.7Å resolution

Structure of the ARTT motif Q212A mutant C3bot1 Exoenzyme (NAD-bound state, crystal form I)

Released:
DOI: 10.2210/pdb2c8c/pdb
PDB entry 2c8c coloured by chain, front view.
PDB entry 2c8c coloured by chain, side view.
PDB entry 2c8c coloured by chain, top view.
Source organism: Clostridium botulinum
Primary publication:
Structural basis for the NAD-hydrolysis mechanism and the ARTT-loop plasticity of C3 exoenzymes.
Ménétrey J, Flatau G, Boquet P, Ménez A, Stura EA
Protein Sci 17 878-86 (2008)
PMID: 18369192

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exoenzyme C3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 211 amino acids
Theoretical weight: 23.53 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7M0L1 (Residues: 1-18, 19-22; Coverage: 96%)
Structure domains: Toxin ADP-ribosyltransferase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand NAD 3 x NAD
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2
Unit cell:
a: 106.048Å b: 76.135Å c: 120.432Å
α: 90° β: 102.74° γ: 90°
R-values:
R R work R free
0.243 0.241 0.283
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Clostridial toxins.
Popoff et al. (2009)
3 more

2 mentions without citation

Structural basis for the NAD-hydrolysis mechanism and the ARTT-loop plasticity of C3 exoenzymes.
Ménétrey et al. (2008)
1 more