› 2c9k

X-ray diffraction
2.8Å resolution

Structure of the functional form of the mosquito-larvicidal Cry4Aa toxin from Bacillus thuringiensis at 2.8 A resolution

Released:
DOI: 10.2210/pdb2c9k/pdb
PDB entry 2c9k coloured by chain, front view.
PDB entry 2c9k coloured by chain, side view.
PDB entry 2c9k coloured by chain, top view.
Source organism: Bacillus thuringiensis
Primary publication:
Structure of the functional form of the mosquito larvicidal Cry4Aa toxin from Bacillus thuringiensis at a 2.8-angstrom resolution.
Boonserm P, Mo M, Angsuthanasombat C, Lescar J
J Bacteriol 188 3391-401 (2006)
PMID: 16621834

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147746 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pesticidal crystal protein Cry4Aa Chain: A
Molecule details ›
Chain: A
Length: 612 amino acids
Theoretical weight: 69.9 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: P16480 (Residues: 68-679; Coverage: 52%)
Gene names: cry4Aa, cryIVA(a), isrH4
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 91.199Å b: 202.069Å c: 98.726Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.259
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control.
Bravo et al. (2007)
17 more

12 mentions without citation

Structural insights into Bacillus thuringiensis Cry, Cyt and parasporin toxins.
Xu et al. (2014)
11 more