› 2cbx

X-ray diffraction
2Å resolution

X-ray crystal structure of 5'-fluorodeoxyadenosine synthase from Streptomyces cattleya complexed with beta-D-erythrofuranosyl- adenosine

Released:
DOI: 10.2210/pdb2cbx/pdb
PDB entry 2cbx coloured by chain, front view.
PDB entry 2cbx coloured by chain, side view.
PDB entry 2cbx coloured by chain, top view.
Source organism: Streptomyces cattleya
Primary publication:
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates.
Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA
Org Biomol Chem 4 1458-60 (2006)
PMID: 16604208

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + fluoride = 5'-deoxy-5'-fluoroadenosine + L-methionine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 299 amino acids
Theoretical weight: 32.4 KDa
Source organism: Streptomyces cattleya
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q70GK9 (Residues: 1-299; Coverage: 100%)
Gene name: flA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CC5 3 x CC5
Ligand GOL 9 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: C2221
Unit cell:
a: 74.815Å b: 128.017Å c: 181.028Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.172 0.239
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Fluoro amino acids: a rarity in nature, yet a prospect for protein engineering.
Odar et al. (2015)
3 more

1 mention without citation

Halogenases: a palette of emerging opportunities for synthetic biology-synthetic chemistry and C-H functionalisation.
Crowe et al. (2021)