› 2ccd

X-ray diffraction
2.1Å resolution

Crystal structure of the catalase-peroxidase (KatG) and S315T mutant from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb2ccd/pdb
PDB entry 2ccd coloured by chain, front view.
PDB entry 2ccd coloured by chain, side view.
PDB entry 2ccd coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Hydrogen peroxide-mediated isoniazid activation catalyzed by Mycobacterium tuberculosis catalase-peroxidase (KatG) and its S315T mutant.
Zhao X, Yu H, Yu S, Wang F, Sacchettini JC, Magliozzo RS
Biochemistry 45 4131-40 (2006)
PMID: 16566587

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase-peroxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 740 amino acids
Theoretical weight: 80.7 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WIE5 (Residues: 1-740; Coverage: 100%)
Gene names: MTCY180.10, Rv1908c, katG
Sequence domains: Peroxidase
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P42212
Unit cell:
a: 149.807Å b: 149.807Å c: 154.493Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.276
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Evolution of catalases from bacteria to humans.
Zamocky et al. (2008)
13 more

4 mentions without citation

Comparative Genome and Network Centrality Analysis to Identify Drug Targets of Mycobacterium tuberculosis H37Rv.
Melak et al. (2015)
3 more