› 2cdh

X-ray diffraction
4.2Å resolution

ARCHITECTURE OF THE THERMOMYCES LANUGINOSUS FUNGAL FATTY ACID SYNTHASE AT 5 ANGSTROM RESOLUTION.

Released:
DOI: 10.2210/pdb2cdh/pdb
PDB entry 2cdh coloured by chain, front view.
PDB entry 2cdh coloured by chain, side view.
PDB entry 2cdh coloured by chain, top view.
Source organism: Thermomyces lanuginosus
Primary publication:
Architecture of a fungal fatty acid synthase at 5 A resolution.
Jenni S, Leibundgut M, Maier T, Ban N
Science 311 1263-7 (2006)
PMID: 16513976

Function and Biology Details

Reactions catalysed: 
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero 36-mer (preferred)
PDBe Complex ID:
PDB-CPX-141814 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
ENOYL REDUCTASE Chains: 0, 1, 2, 3, Y, Z
Molecule details ›
Chains: 0, 1, 2, 3, Y, Z
Length: 226 amino acids
Theoretical weight: 24.09 KDa
Source organism: Thermomyces lanuginosus
MALONYL/PALMITOYL TRANSFERASE Chains: 4, 5, 6, 7, 8, 9, M, N, O, P, Q, R
Molecule details ›
Chains: 4, 5, 6, 7, 8, 9, M, N, O, P, Q, R
Length: 305 amino acids
Theoretical weight: 31.12 KDa
Source organism: Thermomyces lanuginosus
3-oxoacyl-[acyl-carrier-protein] synthase 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 406 amino acids
Theoretical weight: 42.68 KDa
Source organism: Thermomyces lanuginosus
UniProt:
  • Canonical: P0A953 (Residues: 1-406; Coverage: 100%)
Gene names: JW2320, b2323, fabB, fabC
Sequence domains:
3-oxoacyl-[acyl-carrier-protein] reductase 1, chloroplastic Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 244 amino acids
Theoretical weight: 25.36 KDa
Source organism: Thermomyces lanuginosus
UniProt:
  • Canonical: Q93X62 (Residues: 77-320; Coverage: 76%)
Gene name: gbkr1
Sequence domains: short chain dehydrogenase
DEHYDRATASE Chains: S, T, U, V, W, X
Molecule details ›
Chains: S, T, U, V, W, X
Length: 248 amino acids
Theoretical weight: 27.62 KDa
Source organism: Thermomyces lanuginosus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 217Å b: 415Å c: 222Å
α: 90° β: 111.5° γ: 90°

Quick links

Citations

22 review citations

Current understanding of fatty acid biosynthesis and the acyl carrier protein.
Chan et al. (2010)
21 more