› 2cdm

X-ray diffraction
2.7Å resolution

The structure of TrwC complexed with a 27-mer DNA comprising the recognition hairpin and the cleavage site

Released:
DOI: 10.2210/pdb2cdm/pdb
PDB entry 2cdm coloured by chain, front view.
PDB entry 2cdm coloured by chain, side view.
PDB entry 2cdm coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Unveiling the molecular mechanism of a conjugative relaxase: The structure of TrwC complexed with a 27-mer DNA comprising the recognition hairpin and the cleavage site.
Boer R, Russi S, Guasch A, Lucas M, Blanco AG, Pérez-Luque R, Coll M, de la Cruz F
J Mol Biol 358 857-69 (2006)
PMID: 16540117

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115147 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
TrwC relaxase domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 293 amino acids
Theoretical weight: 33.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47673 (Residues: 1-293; Coverage: 30%)
Gene name: trwC
Sequence domains: TrwC relaxase
5'-D(*GP*CP*GP*CP*AP*CP*CP*GP*AP*AP *AP*GP*GP*TP*GP*CP*GP*TP*AP*TP*TP*GP*TP*CP*TP*AP*T)-3' Chains: B, D
Molecule details ›
Chains: B, D
Length: 27 nucleotides
Theoretical weight: 8.33 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P65
Unit cell:
a: 148.383Å b: 148.383Å c: 79.505Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.247
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

11 review citations

Biological diversity of prokaryotic type IV secretion systems.
Alvarez-Martinez et al. (2009)
10 more

6 mentions without citation

Breaking and joining single-stranded DNA: the HUH endonuclease superfamily.
Chandler et al. (2013)
5 more