› 2cf5

X-ray diffraction
2Å resolution

Crystal Structures of the Arabidopsis Cinnamyl Alcohol Dehydrogenases, AtCAD5

Released:
DOI: 10.2210/pdb2cf5/pdb
PDB entry 2cf5 coloured by chain, front view.
PDB entry 2cf5 coloured by chain, side view.
PDB entry 2cf5 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structures and catalytic mechanism of the Arabidopsis cinnamyl alcohol dehydrogenases AtCAD5 and AtCAD4.
Youn B, Camacho R, Moinuddin SG, Lee C, Davin LB, Lewis NG, Kang C
Org Biomol Chem 4 1687-97 (2006)
PMID: 16633561

Function and Biology Details

Reaction catalysed: 
Cinnamyl alcohol + NADP(+) = cinnamaldehyde + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129272 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cinnamyl alcohol dehydrogenase 5 Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 38.79 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O49482 (Residues: 1-357; Coverage: 100%)
Gene names: At4g34230, CAD-D, CAD5, CAD6, F28A23.10, LCAD-D
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41212
Unit cell:
a: 54.22Å b: 54.22Å c: 312.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.235
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Dissection of lignin macromolecular configuration and assembly: comparison to related biochemical processes in allyl/propenyl phenol and lignan biosynthesis.
Davin et al. (2008)
2 more

6 mentions without citation

Structure and function of enzymes involved in the biosynthesis of phenylpropanoids.
Ferrer et al. (2008)
5 more