› 2cfq

X-ray diffraction
2.95Å resolution

Sugar Free Lactose Permease at neutral pH

Released:
DOI: 10.2210/pdb2cfq/pdb
PDB entry 2cfq coloured by chain, front view.
PDB entry 2cfq coloured by chain, side view.
PDB entry 2cfq coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY.
Mirza O, Guan L, Verner G, Iwata S, Kaback HR
EMBO J 25 1177-83 (2006)
PMID: 16525509

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactose permease Chain: A
Molecule details ›
Chain: A
Length: 417 amino acids
Theoretical weight: 46.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02920 (Residues: 1-417; Coverage: 100%)
Gene names: JW0334, b0343, lacY
Sequence domains: LacY proton/sugar symporter
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

1 bound ligand:
Ligand HG 5 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P43212
Unit cell:
a: 122.47Å b: 122.47Å c: 187.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.262 0.262 0.298
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Insights into Protein-Ligand Interactions: Mechanisms, Models, and Methods.
Du et al. (2016)
14 more

43 mentions without citation

The alternating access transport mechanism in LacY.
Kaback et al. (2011)
42 more