2cgp

X-ray diffraction
2.2Å resolution

CATABOLITE GENE ACTIVATOR PROTEIN/DNA COMPLEX, ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

Released:
Source organism: Escherichia coli
Primary publication:
The structure of a CAP-DNA complex having two cAMP molecules bound to each monomer.
Proc Natl Acad Sci U S A 94 2843-7 (1997)
PMID: 9096308

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-115148 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding transcriptional dual regulator CRP Chain: A
Molecule details ›
Chain: A
Length: 210 amino acids
Theoretical weight: 23.67 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 1-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:
DNA (5'-D(*GP*TP*CP*AP*CP*AP*TP*TP*AP*AP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.33 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(*AP*TP*TP*AP*AP*TP*GP*TP*GP*AP*CP*AP*TP*AP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.61 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P3121
Unit cell:
a: 79.19Å b: 79.19Å c: 140.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 not available 0.296
Expression system: Not provided