› 2ci5

X-ray diffraction
1.79Å resolution

Crystal structure of Dimethylarginine Dimethylaminohydrolase I in complex with L-homocysteine

Released:
DOI: 10.2210/pdb2ci5/pdb
PDB entry 2ci5 coloured by chain, front view.
PDB entry 2ci5 coloured by chain, side view.
PDB entry 2ci5 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure of the mammalian NOS regulator dimethylarginine dimethylaminohydrolase: A basis for the design of specific inhibitors.
Frey D, Braun O, Briand C, Vasák M, Grütter MG
Structure 14 901-11 (2006)
PMID: 16698551

Function and Biology Details

Reaction catalysed: 
N(omega),N(omega)-dimethyl-L-arginine + H(2)O = dimethylamine + L-citrulline
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 31.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P56965 (Residues: 2-285; Coverage: 100%)
Gene name: DDAH1
Sequence domains: Arginine deiminase
Structure domains: L-arginine/glycine Amidinotransferase; Chain A

Ligands and Environments

2 bound ligands:
Ligand HCS 2 x HCS
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 44.603Å b: 79.363Å c: 78.617Å
α: 90° β: 104.8° γ: 90°
R-values:
R R work R free
0.176 0.176 0.211

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Citations

12 review citations

Dimethylarginine dimethylaminohydrolase (DDAH): expression, regulation, and function in the cardiovascular and renal systems.
Palm et al. (2007)
11 more

1 mention without citation

Specific reactions of S-nitrosothiols with cysteine hydrolases: A comparative study between dimethylargininase-1 and CTP synthetase.
Braun et al. (2007)