› 2ckb

X-ray diffraction
3Å resolution

STRUCTURE OF THE 2C/KB/DEV8 COMPLEX

Released:
DOI: 10.2210/pdb2ckb/pdb
PDB entry 2ckb coloured by chain, front view.
PDB entry 2ckb coloured by chain, side view.
PDB entry 2ckb coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis of plasticity in T cell receptor recognition of a self peptide-MHC antigen.
Garcia KC, Degano M, Pease LR, Huang M, Peterson PA, Teyton L, Wilson IA
Science 279 1166-72 (1998)
PMID: 9469799

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-224972 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
T-cell receptor alpha chain V region PHDS58 Chains: A, C
Molecule details ›
Chains: A, C
Length: 202 amino acids
Theoretical weight: 22.3 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01738 (Residues: 21-130; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
ALPHA, BETA T CELL RECEPTOR Chains: B, D
Molecule details ›
Chains: B, D
Length: 237 amino acids
Theoretical weight: 26.28 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
Structure domains: Immunoglobulins
H-2 class I histocompatibility antigen, K-B alpha chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 274 amino acids
Theoretical weight: 31.65 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01901 (Residues: 22-295; Coverage: 79%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Cytochrome c oxidase subunit NDUFA4 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 8 amino acids
Theoretical weight: 1.06 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q62425 (Residues: 61-68; Coverage: 10%)
Gene name: Ndufa4
Beta-2-microglobulin Chains: L, M
Molecule details ›
Chains: L, M
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21212
Unit cell:
a: 295.66Å b: 89.96Å c: 84.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.322
Expression systems:
  • Drosophila melanogaster
  • Not provided

Quick links

Citations

124 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
123 more

45 mentions without citation

Evolutionarily conserved amino acids that control TCR-MHC interaction.
Marrack et al. (2008)
44 more