› 2cl3

X-ray diffraction
1.9Å resolution

Crystal structure of human Cleavage and Polyadenylation Specificity Factor 5 (CPSF5)

Released:
DOI: 10.2210/pdb2cl3/pdb
PDB entry 2cl3 coloured by chain, front view.
PDB entry 2cl3 coloured by chain, side view.
PDB entry 2cl3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of human cleavage and polyadenylation specific factor-5 reveals a dimeric Nudix protein with a conserved catalytic site.
Trésaugues L, Stenmark P, Schüler H, Flodin S, Welin M, Nyman T, Hammarström M, Moche M, Gräslund S, Nordlund P
Proteins 73 1047-52 (2008)
PMID: 18767156

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cleavage and polyadenylation specificity factor subunit 5 Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 26.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43809 (Residues: 19-227; Coverage: 92%)
Gene names: CFIM25, CPSF25, CPSF5, NUDT21
Sequence domains: Nucleotide hydrolase
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3121
Unit cell:
a: 79.92Å b: 79.92Å c: 72.22Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.17 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The structure of human cleavage factor I(m) hints at functions beyond UGUA-specific RNA binding: a role in alternative polyadenylation and a potential link to 5' capping and splicing.
Yang et al. (2011)
1 more

12 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
11 more