› 2cmh

X-ray diffraction
2.3Å resolution

Crystal Structure of Spermidine Synthase from Helicobacter Pylori

Released:
DOI: 10.2210/pdb2cmh/pdb
PDB entry 2cmh coloured by chain, front view.
PDB entry 2cmh coloured by chain, side view.
PDB entry 2cmh coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Crystal structure of Helicobacter pylori spermidine synthase: a Rossmann-like fold with a distinct active site.
Lu PK, Tsai JY, Chien HY, Huang H, Chu CH, Sun YJ
Proteins 67 743-54 (2007)
PMID: 17357156

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127760 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyamine aminopropyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 262 amino acids
Theoretical weight: 30.59 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: O25503 (Residues: 1-262; Coverage: 100%)
Gene names: HP_0832, speE
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: C2221
Unit cell:
a: 98.526Å b: 126.468Å c: 143.948Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.27
Expression system: Escherichia coli

Quick links

Citations

3 review citations

A multifaceted role for polyamines in bacterial pathogens.
Shah et al. (2008)
2 more

1 mention without citation

The efficacy of high-flow nasal cannula (HFNC) versus non-invasive ventilation (NIV) in patients at high risk of extubation failure: a systematic review and meta-analysis.
Wang et al. (2023)