› 2cnb

X-ray diffraction
2.7Å resolution

Trypanosoma brucei UDP-galactose-4-epimerase in ternary complex with NAD and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose

Released:
DOI: 10.2210/pdb2cnb/pdb
PDB entry 2cnb coloured by chain, front view.
PDB entry 2cnb coloured by chain, side view.
PDB entry 2cnb coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
Trypanosoma brucei UDP-galactose-4'-epimerase in ternary complex with NAD+ and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose.
Alphey MS, Burton A, Urbaniak MD, Boons GJ, Ferguson MA, Hunter WN
Acta Crystallogr Sect F Struct Biol Cryst Commun 62 829-34 (2006)
PMID: 16946458

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186145 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 4-epimerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 397 amino acids
Theoretical weight: 43.81 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8T8E9 (Residues: 1-395; Coverage: 100%)
Gene name: galE
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
1 bound ligand:
Ligand UFG 4 x UFG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 101.724Å b: 111.694Å c: 160.873Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.199 0.281
Expression system: Escherichia coli BL21

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Citations

10 citation in other articles

Fluorine-protein interactions and ¹⁹F NMR isotropic chemical shifts: An empirical correlation with implications for drug design.
Dalvit et al. (2011)
9 more

4 mentions without citation

Patchwork assembly of nag-like nitroarene dioxygenase genes and the 3-chlorocatechol degradation cluster for evolution of the 2-chloronitrobenzene catabolism pathway in Pseudomonas stutzeri ZWLR2-1.
Liu et al. (2011)
3 more