› 2csb

X-ray diffraction
2.3Å resolution

Crystal structure of Topoisomerase V from Methanopyrus kandleri (61 kDa fragment)

Released:
DOI: 10.2210/pdb2csb/pdb
PDB entry 2csb coloured by chain, front view.
PDB entry 2csb coloured by chain, side view.
PDB entry 2csb coloured by chain, top view.
Source organism: Methanopyrus kandleri
Primary publication:
Structure of the N-terminal fragment of topoisomerase V reveals a new family of topoisomerases.
Taneja B, Patel A, Slesarev A, Mondragón A
EMBO J 25 398-408 (2006)
PMID: 16395333

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Helix-hairpin-helix DNA-binding motif class 1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 519 amino acids
Theoretical weight: 60.33 KDa
Source organism: Methanopyrus kandleri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q977W1 (Residues: 1-519; Coverage: 53%)
Gene names: HA336_03250, top5
Sequence domains:
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 70.69Å b: 89.77Å c: 189.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.292
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

All tangled up: how cells direct, manage and exploit topoisomerase function.
Vos et al. (2011)
12 more

7 mentions without citation

Structural studies of type I topoisomerases.
Baker et al. (2009)
6 more