› 2cun

X-ray diffraction
2.1Å resolution

Crystal structure of Phosphoglycerate Kinase from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb2cun/pdb
PDB entry 2cun coloured by chain, front view.
PDB entry 2cun coloured by chain, side view.
PDB entry 2cun coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Mizutani H, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129787 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoglycerate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 410 amino acids
Theoretical weight: 46.47 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58965 (Residues: 1-410; Coverage: 100%)
Gene names: PH1218, PHBK036, pgk
Sequence domains: Phosphoglycerate kinase
Structure domains: Phosphoglycerate kinase, N-terminal domain

Ligands and Environments

4 bound ligands:
Ligand CL 2 x CL
Ligand 3PG 2 x 3PG
Ligand GOL 2 x GOL
Ligand MPD 3 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 49.854Å b: 123.327Å c: 82.943Å
α: 90° β: 95.42° γ: 90°
R-values:
R R work R free
0.212 0.21 0.244
Expression system: Escherichia coli

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