› 2cuw

X-ray diffraction
2.3Å resolution

Crystal Structure of Thermus thermophilus PurS, one of the subunits of Formylglycinamide Ribonucleotide Amidotransferase in the purine biosynthetic pathway

Released:
DOI: 10.2210/pdb2cuw/pdb
PDB entry 2cuw coloured by chain, front view.
PDB entry 2cuw coloured by chain, side view.
PDB entry 2cuw coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Yanai H, Kanagawa M, Sampei G, Kawai G, Yokoyama S, Kuramitsu S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H(2)O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-177933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylformylglycinamidine synthase subunit PurS Chain: A
Molecule details ›
Chain: A
Length: 84 amino acids
Theoretical weight: 9.36 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SI58 (Residues: 1-84; Coverage: 100%)
Gene names: TTHA1516, purS
Sequence domains: Phosphoribosylformylglycinamidine (FGAM) synthase
Structure domains: Phosphoribosylformylglycinamidine synthase subunit PurS

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2
Unit cell:
a: 59.72Å b: 49.38Å c: 43.75Å
α: 90° β: 133.56° γ: 90°
R-values:
R R work R free
0.235 0.231 0.275
Expression system: Escherichia coli

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