› 2cuz

X-ray diffraction
1.98Å resolution

Glutamyl-tRNA synthetase from Thermus thermophilus in complex with L-glutamate

Released:
DOI: 10.2210/pdb2cuz/pdb
PDB entry 2cuz coloured by chain, front view.
PDB entry 2cuz coloured by chain, side view.
PDB entry 2cuz coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Structural bases of transfer RNA-dependent amino acid recognition and activation by glutamyl-tRNA synthetase.
Sekine S, Shichiri M, Bernier S, Chênevert R, Lapointe J, Yokoyama S
Structure 14 1791-9 (2006)
PMID: 17161369

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150904 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 468 amino acids
Theoretical weight: 53.99 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27000 (Residues: 1-468; Coverage: 100%)
Gene names: TTHA0438, gltX
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GLU 1 x GLU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 82.152Å b: 84.04Å c: 83.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.228 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Experimental and computational determination of tRNA dynamics.
Alexander et al. (2010)
1 more

3 mentions without citation

Personalized Medicine in Mitochondrial Health and Disease: Molecular Basis of Therapeutic Approaches Based on Nutritional Supplements and Their Analogs.
Tragni et al. (2022)
2 more