› 2cv1

X-ray diffraction
2.41Å resolution

Glutamyl-tRNA synthetase from Thermus thermophilus in complex with tRNA(Glu), ATP, and an analog of L-glutamate: a quaternary complex

Released:
DOI: 10.2210/pdb2cv1/pdb
PDB entry 2cv1 coloured by chain, front view.
PDB entry 2cv1 coloured by chain, side view.
PDB entry 2cv1 coloured by chain, top view.
Source organism: Thermus thermophilus
Entry authors: Sekine S, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150909 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Glutamate--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 468 amino acids
Theoretical weight: 53.99 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27000 (Residues: 1-468; Coverage: 100%)
Gene names: TTHA0438, gltX
Sequence domains:
Structure domains:
tRNA Chains: C, D
Molecule details ›
Chains: C, D
Length: 75 nucleotides
Theoretical weight: 24.11 KDa
Source organism: Thermus thermophilus
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand ATP 2 x ATP
Ligand GAU 2 x GAU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C2221
Unit cell:
a: 110.624Å b: 218.818Å c: 134.858Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.277
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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