› 2cv4

X-ray diffraction
2.3Å resolution

Crystal Structure of an Archaeal Peroxiredoxin from the Aerobic Hyperthermophilic Crenarchaeon Aeropyrum pernix K1

Released:
DOI: 10.2210/pdb2cv4/pdb
PDB entry 2cv4 coloured by chain, front view.
PDB entry 2cv4 coloured by chain, side view.
PDB entry 2cv4 coloured by chain, top view.
Source organism: Aeropyrum pernix K1
Primary publication:
Crystal structure of an archaeal peroxiredoxin from the aerobic hyperthermophilic crenarchaeon Aeropyrum pernix K1.
Mizohata E, Sakai H, Fusatomi E, Terada T, Murayama K, Shirouzu M, Yokoyama S
J Mol Biol 354 317-29 (2005)
PMID: 16214169

Function and Biology Details

Reaction catalysed: 
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195428 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxiredoxin Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 250 amino acids
Theoretical weight: 29.03 KDa
Source organism: Aeropyrum pernix K1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y9L0 (Residues: 1-250; Coverage: 100%)
Gene name: APE_2278
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MES 2 x MES
Ligand IPA 9 x IPA
2 modified residues:
Ligand MSE 50 x MSE
Ligand OCS 10 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P1
Unit cell:
a: 76.738Å b: 103.434Å c: 102.814Å
α: 105.18° β: 92.91° γ: 105.39°
R-values:
R R work R free
0.178 0.178 0.23
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The oligomeric conformation of peroxiredoxins links redox state to function.
Barranco-Medina et al. (2009)
6 more

6 mentions without citation

Structure-based insights into the catalytic power and conformational dexterity of peroxiredoxins.
Hall et al. (2011)
5 more