› 2cvt

X-ray diffraction
3.2Å resolution

Structures of Yeast Ribonucleotide Reductase I

Released:
DOI: 10.2210/pdb2cvt/pdb
PDB entry 2cvt coloured by chain, front view.
PDB entry 2cvt coloured by chain, side view.
PDB entry 2cvt coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structures of eukaryotic ribonucleotide reductase I provide insights into dNTP regulation.
Xu H, Faber C, Uchiki T, Fairman JW, Racca J, Dealwis C
Proc Natl Acad Sci U S A 103 4022-7 (2006)
PMID: 16537479

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase large chain 1 Chain: A
Molecule details ›
Chain: A
Length: 888 amino acids
Theoretical weight: 99.67 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21524 (Residues: 1-888; Coverage: 100%)
Gene names: CRT7, RIR1, RNR1, SDS12, YER070W
Sequence domains:
Structure domains: Anaerobic Ribonucleotide-triphosphate Reductase Large Chain

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P21212
Unit cell:
a: 110.253Å b: 117.071Å c: 63.337Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.226 0.298
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

DNA building blocks: keeping control of manufacture.
Hofer et al. (2012)
8 more

1 mention without citation

Structures of eukaryotic ribonucleotide reductase I provide insights into dNTP regulation.
Xu et al. (2006)